Comparison of autoclaving and γ-radiation impact on four spices aroma profiles and microbial load using HS-SPME GC-MS and chemometric tools.

Herbal spices are widely consumed as food additives owing to their distinct aroma and taste as well as a myriad of economic and health value. The aroma profile of four major spices including bay leaf, black pepper, capsicum, and fennel was tested using HS-SPME/GC-MS and in response to the most widely used spices´ processing methods including autoclaving and γ-radiation at low and high doses. Additionally, the impact of processing on microbial contamination of spices was tested using total aerobic count. GC-MS analysis led to the identification of 22 volatiles in bay leaf, 34 in black pepper, 23 in capsicum, and 24 in fennel. All the identified volatiles belonged to oxides/phenols/ethers, esters, ketones, alcohols, sesquiterpene and monoterpene hydrocarbons. Oxides/phenol/ethers were detected at high levels in all tested spices at ca. 44, 28.2, 48.8, 61.1%, in bay leaves, black pepper, capsicum, and fennel, respectively of the total blend and signifying their typical use as spices. Total oxides/phenol/ethers showed an increase in bay leaf upon exposure to γ-radiation from 44 to 47.5%, while monoterpene hydrocarbons were enriched in black pepper upon autoclaving from 11.4 in control to reach 65.9 and 82.6% for high dose and low dose of autoclaving, respectively. Cineole was detected in bay leaf at 17.9% and upon exposure to autoclaving at high dose and γ-radiation (both doses) its level increased by 29-31%. Both autoclaving and γ-radiation distinctly affected aroma profiles in examined spices. Further, volatile variations in response to processing were assessed using multivariate data analysis (MVA) revealing distinct separation between autoclaved and γ-radiated samples compared to control. Both autoclaving at 115 °C for 15 min and radiation at 10 kGy eliminated detected bioburden in all tested spices i.e., reduced the microbial counts below the detection limit (< 10 cfu/g).

Sequential electron beam and bioflocculation for treatment of textile nanodyes.

Nanodyes are a new class of hazardous materials that are used in textile coloring. Their small size, color, stability and high dispersion characteristics pose a huge threat if they are released in open water systems. The aim of the present study is to test electron beam irradiation, bioflocculation and their sequential use for nanodye removal. The nanodye was obtained from a factory and was characterized using UV-visible spectroscopy, Fourier transform infra-red (FTIR) spectroscopy, dynamic light scattering, zeta potential and energy dispersive X-ray (EDX). The obtained results show that applying 7.5 kGy electron beam irradiation results in complete color removal in 10 min for 50 and 100 ppm nanodye, while at 200 and 400 ppm concentrations, the decolorization reaches 90% but leaving a residual brownish color. Adding 5 mg mL-1 of Serratia marcescens N2 biosurfactant resulted in agglomeration of 80% dye removal for 400 ppm nanodye after 24 h. On the other hand, the use of sequential electron beam and bioflocculation led to an initial removal of 80% in 1 h. The residual dyes were tested for toxicity on normal dermal HFB4 cells. The toxicity result was 1.19% after electron beam treatment, while those for sequential treatment and bioflocculation were 6.28 and 6.9%, respectively. It can be concluded that electron beam technology provides fast and highly efficient nanodye removal, while biosurfactants offer a low-cost, eco-friendly approach with a chance for dye retrieval.

Valorization of frying oil waste for biodetergent production using Serratia marcescens N2 and gamma irradiation assisted biorecovery.

BACKGROUND: The complexity, toxicity and abundance of frying oil waste (FOW) render it difficult to be degraded biologically. The aim of the present work was to valorize FOW and investigate the potential use of the produced biosurfactant by Serratia marcescens N2 (Whole Genome sequencing accession ID SPSG00000000) as a biodetergent. RESULTS: Serratia marcescens N2 demonstrated efficient valorization of FOW, using 1% peptone, 20% FOW and 8% inoculum size. Gene annotation showed the presence of serrawettin synthetase indicating that the produced biosurfactant was serrawettin. Zeta potential and Fourier Transform Infrared (FTIR) spectroscopy indicate that the biosurfactant produced was a negatively charged lipopeptide. The biosurfactant reduced the surface tension of water from 72 to 25.7 mN/m; its emulsification index was 90%. The valorization started after 1 h of incubation and reached a maximum of 83.3%. Gamma radiation was used to increase the biosurfactant yield from 9.4 to 19.2 g/L for non-irradiated and 1000 Gy irradiated cultures, respectively. It was noted that the biorecovery took place immediately as opposed to overnight storage required in conventional biosurfactant recovery. Both chemical and functional characteristics of the radiation induced biosurfactant did not change at low doses. The produced biosurfactant was used to wash oil stain; the highest detergency reached was 87% at 60 °C under stirring conditions for 500 Gy gamma assisted biorecovery. Skin irritation tests performed on experimental mice showed no inflammation. CONCLUSION: This study was able to obtain a skin friendly effective biodetergent from low worth FOW using Serratia marcescens N2 with 83% efficient valorization using only peptone in the growth media unlike previous studies using complex media. Gamma radiation was for the first time experimented to assist biosurfactant recovery and doubling the yield without affecting the efficiency.

Development of gamma irradiated SSD-embedded hydrogel dyed with prodigiosin as a smart wound dressing: Evaluation in a MDR infected burn rat model.

Burn wounds are prone to infections and cause a large amount of death worldwide. Although burn wound is sterile at the beginning but due to the risk factors such as prolonged hospital stay, immune suppression and burn affecting large surface area, it turns to infected burn. This study aims to examine the possible protective properties of silver sulfadiazine embedded in alginate macromolecule to prepare hydrogel. The prepared hydrogel dyed with prodigiosin was used as a smart wound dressing to treat burn wounds that were infected with S. aureus and E. coli. Rats were divided into four groups: (1) control group, (2) burn-infected with S. aureus and E. coli group, (3) burn-infected treated with silver nitrate cream group and (4) burn-infected gamma irradiated silver sulfadiazine-embedded hydrogel dyed with prodigiosin. The biochemical results verified the histopathological results and our findings showed that silver sulfadiazine-embedded hydrogel dyed with prodigiosin is an effective product in compared with silver nitrate cream.

Green synthesis of novel antifungal 1,2,4-triazoles effective against γ-irradiated Candida parapsilosis.

This study reports the green synthesis of 11 novel 3-substituted-4-amino-5-mercapto-1,2,4-triazole derivatives using water as a readily available nontoxic solvent. Evaluation of their antimicrobial potential against several clinical pathogenic microorganisms was carried out. The newly synthesized cysteine derivative 6 showed promising antifungal activity against both γ-irradiated and nonirradiated Candida parapsilosis 216, with the lowest MIC (minimum inhibitory concentration) value of 3.125 µg/ml, probably through inhibition of 14α-demethylase. In addition, compound 6 showed complete inhibition of gelatinase, a virulence enzyme of C. parapsilosis. Also, scanning electron microscopy was carried out. Interestingly, compound 6 acted as a dual agent as it also showed good antibacterial activity against strains of Gram-positive bacteria used in the study. The synthesized compounds showed no cytotoxicity.

Draft genome sequence of a prodigiosin-hyperproducing Serratia marcescens strain isolated from Cairo, Egypt.

Serratia marcescens is a Gram-negative bacterium with both environmental and host-associated strains. Pigmentation is reportedly inversely correlated with infection frequency, and prodigiosin is one of Serratia pigments that has medical and industrial applications. Here, we report the draft genome sequence of prodigiosin-hyperproducing Serratia marcescens strain N2, isolated from Cairo, Egypt. The sequence is assembled into 142 contigs, with a combined size of 5,570,793 bp. The assembled genome carries typical S. marcescens genes, with potential prodigiosin-biosynthesizing genes detected.

Optimization of prodigiosin production by Serratia marcescens using crude glycerol and enhancing production using gamma radiation.

Prodigiosin is a red pigment produced by Serratia marcescens. Prodigiosin is regarded as a promising drug owing to its reported characteristics of possessing anti-microbial, anti-cancer, and immunosuppressive activity. A factorial design was applied to generate a set of 32 experimental combinations to study the optimal conditions for pigment production using crude glycerol obtained from local biodiesel facility as carbon source for the growth of Serratia marcescens. The maximum production (870 unit/cell) was achieved at 22 °C, at pH 9 with the addition of 1% (w/v) peptone and 109 cell/ml inoculum size after 6 days of incubation. Gamma radiation at dose 200 Gy was capable of doubling the production of the pigment using the optimized conditions and manipulating production temperature. Our results indicate that we have designed an economic medium supporting enhanced Serratia marcescens MN5 prodigiosin production giving an added value for crude glycerol obtained from biodiesel industry.

Laccase production by Pleurotus ostreatus and its application in synthesis of gold nanoparticles.

In this work, the production of fungal laccase was optimized from local isolate of Pleurotus ostreatus using solid state fermentation. Factorial design was used to study the effect of several nutrients on enzyme production. Purification and characterization of the enzyme and the effect of temperature, pH and gamma radiation on fungal growth and enzyme production was investigated. Optimization of production conditions yielded an enzyme with activity over 32,450 IU/g of fermented substrate. Factorial design was capable of establishing the conditions that multiplied the activity of the enzyme several folds, consequently, reducing the cost of production. The enzyme was capable of decolorizing several dyes with over 80% reduction in color confirming the aromatic degrading capability of laccase. The enzyme was also used in the synthesis of gold nanoparticles, proving that laccase from Pleurotus ostreatus has a strong potential in several industrial applications.